ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
ISOLATION AND CULTURE OF MESOPHYLL PROTOPLASTS OF GRAPE (VITIS VINIFERA L.), CV. TREBBIANO DI ROMAGNA |
| Author: | G. Marino |
| Abstract:
Protoplasts were isolated from leaves of in vitro rooted shoots. Cultures gave high yields of protoplasts when incubated in the enzyme solution at 28°C for 18 h in darkness. Initial cell division occurred after 1 week in protoplasts plated at 5 x 104/ml in gelled media, particularly in zeatin-enriched media, and cultured in darkness at 25 ± 1°C. After 20 days only occasional divisions were found in liquid media. In contrast, growing cell-colonies were visible in solid media. | |
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