ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
POLYAMINE UPTAKE IN PROTOPLASTS AND PLANT ORGANELLES |
| Authors: | N. Bagni, R. Pistocchi |
| Abstract:
Recently evidence has increased for both short and long distance transport of polyamines in higher plants. Most of the polyamines taken up by the cells are absorbed by the cell wall, except putrescine. Due to these interferences with the cell wall, the need arose to study uptake into protoplasts. Protoplasts and vacuoles accumulated polyamines within 1 to 2 minutes. A compartmentation analysis showed that exogenously applied spermidine could be recovered inside the cells. The study of the subcellular uptake of spermidine was extended to mitochondria isolated from tubers of Helianthus tuberosus. The results indicated that accumulation of spermidine inside the mitochondria was dependent on the energized state of the membrane and driven by transmembrane Δ  .
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