ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
CELL ENGINEERING BY PROTOPLAST FUSION: IMPLICATIONS FOR PLANT IMPROVEMENT |
| Authors: | A. Zelcer, S. Izhar, D. Wolf, B. Levy, O. Soferman |
| Abstract:
The transfer of cytoplasmic genomes from Lycopersicon and Solanum species to L. esculentum was attempted by means of the "donor-recipient" method. A cytoplasmic-albino Large Red Cherry mutant (ALRC) was utilized as the recipient genotype. Protoplasts from cytoplasm-donor genotypes were irradiated with X-rays, in order to induce chromosome elimination and transfer of cytoplasmic genophores upon fusion. Putative cybrid colonies were isolated and subsequently regenerated. Characterization of ALRC x L. peruvianum cybrids revealed isozyme patterns identical to L. esculentum.. Cybrid plants exhibit a recombinant mt-DNA pattern, containing parental as well as novel fragments. | |
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