ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
ORGANOGENESIS AND SOMATIC EMBRYOGENESIS FROM CALLUS OF WINGED BEAN (PSOPHOCARPUS TETRAGONOLOBUS (L.) DC.) |
| Authors: | S. Venketeswaran, M.A.D.L. Dias, Ursula V. Weyers |
| Abstract:
Callus tissue and suspension cultures of several cultivars of winged bean, Psophocarpus tetragonolobus (L.) DC, were established from hypocotyls, cotyledons, and epicotyl explants. Callus growth was observed best in Murashige-Skoog (MS) salt-sucrose culture medium supplemented with 1 mg/l each of 2,4-dichlorophenoxyacetic acid (2,4-D) and naphthaleneacetic acid (NAA) and 1 mg/l each of NAA and kinetin or 6-benzyladenine (BA). Callus clumps when transferred to a MS medium supplemented with 1 mg/l BA with or without kinetin produced shoots and upon transfer to a medium with 1 mg/l indoleacetic acid (IAA) produced roots to give plantlets. Somatic embryogenesis was obtained by transferring newly initiated calli maintained in a medium with 0.1 – 5.0 mg/l 2,4-D, 2 mg/l each of 2,4-D and NAA or 5 mg/l NAA to a medium free of hormones, supplemented with full or half strength MS salts. Three varieties of winged bean, TPt-1, TPt-2 and UPS-122 responded to form somatic embryos or embryo-like structures in liquid suspension cultures. | |
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