ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
LONG-TERM SOMATIC EMBRYO PRODUCTION AND PLANT REGENERATION FROM EMBRYO-DERIVED PEACH CALLUS |
| Authors: | R. Scorza, J.M. Cordts, S. Mante |
| Abstract:
Habituated embryogenic peach callus cultures have continuously produced somatic embryos and shoot-like structures for over 4 years. Abscisic acid (ABA) at 5 μ M increased embryo production but did not improve shoot production. After 18 months of culture on ½ strength Murashige and Skoog medium without growth regulators, 0.1 μM indolebutyric acid with 5 μ M benzyladenine induced 10% of the calli to form shoots. After 4 years of culture shoot production was sporadic. Embryogenic callus stored for up to 55 days at 4°C retained its embryogenic capacity. Non-embryogenic sectors of callus were found to have lower levels of ABA, dihydrozeatin riboside and trans-zeatin riboside than embryogenic sectors. Growth of embryogenic cultures was improved by the use of Driver-Kuniyuki-Walnut medium in place of Murashige and Skoog medium and by the use of Phytagel (an agar substitute) in place of TC Agar. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
