ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
SYNTHETIC SEED TECHNOLOGY: IMPROVING DESICCATION TOLERANCE OF SOMATIC EMBRYOS OF CELERY |
| Authors: | Y. Kim, J. Janick |
| Abstract:
Density separation of celery somatic embryos (Apium graveolens cv. Utah Improved #15) cultured in liquid medium was achieved with layered sucrose gradients (9 to 40%). Embryos located in the interface between 9 and 12% sucrose had the highest fatty acid content and the highest survival after 24 hr desiccation at 90% relative humidity. Desiccation tolerance was increased by supplementation of embryo production medium with 10-6M abscisic acid (ABA) or 1.0 mM proline. Highest survival was achieved by a combination of ABA plus proline. ABA increased the accumulation and altered the distribution of fatty acids in somatic embryos. | |
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