ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
IN VITRO CLONAL PROPAGATION OF STRAWBERRY FROM IMMATURE ACHENES |
| Author: | E.K. Lis |
| Abstract:
Strawberry achenes Fragaria grandiflora cvs. Redgauntlet and Senga Sengana were separated from receptacles of various ages, wounded by cutting and cultured on Lee and de Fossard (1977) basal medium supplemented with BA and 2,4-D or NAA. Achenes at 11 to 21 days after anthesis regenerated multiple shoots that could be rooted and developed into normal plants within four months after culturing. Concentrations of 2,4-D (0.1, 0.4 or 1.0 mg l-1) together with BA (0.8 or 1.0 mg l-1) at 2% sucrose were used to initiate the culture of achenes at 11 days after anthesis. Shoot regeneration proceeded when the concentration of 2,4-D was reduced to 0.01 mg l-1 or replaced by 0.1 mg l-1 NAA with 0.5 or 0.8 mg l-1 BA and sucrose lowered to 1.5%. This reduced concentration of hormones was sufficient for shoot initiation and development in the achenes between 13 to 21 days after anthesis. | |
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