ISHS Acta Horticulturae 280: I International Symposium on In Vitro Culture and Horticultural Breeding
ORGANOGENESIS AND EMBRYOGENESIS IN LISIANTHUS RUSSELLIANUS HOOK |
| Authors: | B. Ruffoni, C. Damiano, F. Massabò, P. Esposito |
| Abstract:
A scheme for direct shoot organogenesis and somatic embryogenesis induction is proposed. Leaf fragments were cultured on liquid media containing zeatin, 2iP, or BA in order to obtain regeneration. Cells and cell aggregates from liquid culture were subcultured in the same media. After four weeks shoot regenerations were observed on media with zeatin or low concentrations of 2iP, some globular formation were found on medium with lo mg/l 2iP. Histological analysis indicate that somatic embryos in the early phases of development were produced. Regenerants were proliferated, rooted and acclimatized in greenhouses. | |
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