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ISHS Acta Horticulturae 275: International Symposium on the Culture of Subtropical and Tropical Fruits and Crops

INDUCTION OF EMBRYOGENIC CALLUS FROM FLOWER SHOOT TIPS OF DWARF CAVENDISH BANANA

Author:   M. Fitchet
Abstract:
A nodular white callus was induced in cultured terminal floral apices of Musa Dwarf Cavendish banana plants on Murashige and Tucker (1969) medium supplemented with glycine, nicotinic acid, thiamine-HCl, pyridoxin-HCl, 340 mg/l NaH2PO4, 100 mg/l 1-tyrosine, 30 g/l glucose and 5 mg/l N6-benzylaminopurine. Most floral buds on the explants developed directly into plants, but callus formation was exhibited in 3 % of the cultures. Plantlets were also regenerated from callus on the same medium. The initial stages of this regeneration from callus appeared to be via somatic embryo development.

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