ISHS Acta Horticulturae 273: V International Workshop on Fireblight
EVALUATION OF FIRE BLIGHT RESISTANCE OF SOMACLONAL VARIANTS OBTAINED FROM THE PEAR CULTIVAR “DURONDEAU” |
| Author: | J. Viseur |
| Abstract:
In vitro regeneration of plantlets was obtained from calluses induced on roots, leaves or stems of the pear cultivars "Durondeau", "Conférence" and "Doyenné du Comice". Regenerated plantlets were micropropagated, and their progenies were submitted to an early selection test for resistance toward fireblight caused by Erwinia amylovora. Plants regenerated from calluses initiated on roots of the cv "Durondeau" gave 3 clones with a peculiar behaviour. When inoculated in vitro with E. amylovora, axillary buds developed from the stem of infected plantlets, producing new shoots which escaped infection. After acclimatization, plants of two of the three clones were dwarf, with shorter internodes and slower growth than the original cultivar. Plants from the third clone were similar to the cultivar, except for a more branched structure. When inoculated in the growth chamber with E. amylovora, the dwarf clones showed a higher resistance than the original cultivar. Plants from the two dwarf clones were found to be tetraploïds (chromosome number 2n=68), while the third clone and the cultivar "Durondeau" were diploïds (2n=34). | |
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