ISHS Acta Horticulturae 273: V International Workshop on Fireblight
STRUCTURE OF THE EXTRACELLULAR POLYSACCHARIDE OF ERWINIA AMYLOVORA: A PRELIMINARY REPORT |
| Authors: | A.R.W. Smith, R.A. Rastall, N.H. Rees, R.C. Hignett, R. Wait |
| Abstract:
Extracellular polysaccharide (EPS) of Erwinia amylovora produced during growth in sorbitol asparagine salts medium contained protein, which was removed by phenol/water treatment, and lipopolysaccharide, which was removed by ultracentrifugation and ion exchange chromatography. Purified EPS contained only galactose, glucuronic acid, acetate and pyruvate in molar ratio 4:1:1:1, together with a small quantity (0.2 mol) of glucose. From 1H-NMR spectroscopy, methylation analysis, and evidence from Smith degradation, B-elimination and acetolysis, the most likely structure of deacylated EPS was:
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