ISHS Acta Horticulturae 273: V International Workshop on Fireblight
DNA-HYBRIDIZATION, A SPECIFIC METHOD FOR THE DIAGNOSIS OF FIRE BLIGHT |
| Authors: | B. Steinbrenner, W. Zeller, P. Bellemann, K. Geider |
| Abstract:
The recently published method (Falkenstein et al., 1988) to identify the fire blight pathogen Erwinia amylovora by DNA-hybridization with a radioactive probe was simplified by the use of a non-radioactive procedure. The probe was a cloned SalI-fragment from the 29 kb E. amylovora plasmid, common to all natural isolates. The DNA was labelled with commercially available digoxigenin-substituted deoxyuridine triphosphate, which was developed after hybridization with specific antiserum conjugated with alkaline phosphatase. Compared to the radioactive method, this variant is as specific but less sensitive. Identification of E. amylovora by immunofluorescence is more sensitive than DNA-hybridization but usually less specific. | |
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