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ISHS Acta Horticulturae 272: Symposium on Bedding and Pot Plant Culture

EVALUATION OF LOW TEMPERATURE FLOWER FORMATION IN CHRYSANTHEMUM USING SEVERAL MICROSCOPY TECHNIQUES

Authors:   M.G. Karlsson, H.C.H. McIntyre
Abstract:
The flower formation process at low temperature (10°C) in Dendranthema grandiflora Tzvelev. ‘Bright Golden Anne’ was compared to flower formation at 15°C. The transition from vegetative to reproductive meristem was documented by light, fluorescence, and scanning electron microscopy (SEM) at weekly intervals for 5 weeks. SEM provided the best documentation for the progression of flower formation while the event of flower initiation was most easily detected using a fluorescence microscope or a stereo-microscope. Both the initiation and development of flowers were delayed at 10° compared to 15°C. After 21 short days at 15°C, 7–8 rows of floret primordia were formed on the receptacle. Plants grown at 10°C had formed 4–5 rows of floret primordia after 28 short days.

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