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| Authors: | A.J. Conner, M. K. Williams, Jane E. Lancaster, Martin L. Shaw, Peter G. Falloon, Simon C. Deroles, R. C. Gardner |
Abstract:
The monocotyledonous genus Asparagus is confirmed as a host for Agrobacterium-mediated transformation.
Small tumour-like swellings developed on shoots of Asparagus species following inoculation with A. tumefaciens (strains C58, A722), A. rhizogenes (strains A4, A4T) and A. rubi (strain 13335). Opines characteristic of each strain were detected in extracts from these tumours.
Transformed cell cultures have been selected following in vitro cocultivation of Asparagus officinalis (CRD157) tissue with A. tumefaciens. Using strain C58, hormone independent, nopaline producing callus cultures have been selected.
Cocultivation with C58C1 pGV3850::1103neo allowed the selection and regeneration of kanamycin-resistant cell cultures.
Proof of transformation has involved quantitation of nopaline, detection of neomycin phosphotransferase II activity and Southern analysis.
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