ISHS Acta Horticulturae 271: VII International Asparagus Symposium
VEGETATIVE COMPATIBILITY GROUPINGS AMONG ISOLATES OF FUSARIUM OXYSPORUM AND F. MONILIFORME COLONIZING ASPARAGUS TISSUES |
| Authors: | W.H. Elmer, J.A. LaMondia, C.T. Stephens |
| Abstract:
Over 100 isolates of Fusarium oxysporum (Schlecht) emend. Snyd. & Hans. and more than 200 isolates of F. moniliforme (Sheld.) emend. Snyd. & Hans. were obtained from field grown asparagus tissue in Michigan and Connecticut, or from global collections. All isolates were virulent on asparagus seedlings. Isolates were placed in vegetative compatibility groups (VCGs) by demonstrating heterokaryosis between isolates of the same species. Heterokaryosis was verified by pairing nitrate non-utilizing (nit) mutants from two different isolates on a nitrate medium. Isolates of F. oxysporum were placed into 43 VCGs with no correlation between VCG and location. The largest VCG of F. oxysporum contained strains from Taiwan, Washington, USA and Michigan USA. At present over 20 VCGs of F. moniliforme have been identified among isolates colonizing asparagus. Six VCGs are recovered more frequently than others. These findings indicate that virulence on asparagus may be a common trait among genetically distinct populations of F. moniliforme and F. oxysporum. | |
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