ISHS Acta Horticulturae 271: VII International Asparagus Symposium
METHODS USED TO SCREEN FOR FUSARIUM RESISTANCE IN ASPARAGUS PLANTS REGENERATED FROM PROTOPLASTS. |
| Authors: | T.L. Wacker, M.L. Smither, T.C. Stebbins, C.T. Stephens |
| Abstract:
Protoclones of Asparagus officinalis cv. Jersey Giant were screened for enhanced resistance to Fusarium oxysporum f. sp. asparagi and Fusarium moniliforme. Conidial suspensions (106 conidia/ml) were applied directly to protoclones in Hoagland's or MS media, or sprayed onto paper towels used to hold protoclone roots. Root lesions and normal disease symptoms developed on UC 157 seedlings, the susceptible control, but not on protoclones where fungal hyphae overgrew stems. A greenhouse assay was the most suitable method to screen asparagus protoclones for disease resistance. Protoclones were acclimated to the greenhouse for 2 weeks then transferred to soil infested with Fusarium. Symptoms of root lesions and crown rot developed on UC 157 and the protoclones after 4 weeks. Disease resistance, as measured by a visual rating scale varied but was less than somaclones of A. sprengeri, the resistant control. | |
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