ISHS Acta Horticulturae 266: V International Symposium on Flower Buds
COMPARISONS OF PRECULTURAL TREATMENTS AND CULTURAL CONDITIONS ON IN VITRO RESPONSE OF TULIP |
| Authors: | C.M. Baker, H.F. Wilkins, P.D. Ascher |
| Abstract:
Temperature and duration of bulb storage, type of explant, surface disinfestation method, medium, and culture temperature were compared for effects on in vitro response of explants from ‘Apeldoorn’ bulbs. Explants from scale tissue turned brown whereas those from the floral axis developed leaf-like structures. Bulbs stored for either 56 or 63 days had superior mean visual ratings when compared to bulbs stored for 36 days. Explants placed on MS medium containing NAA + BA (0.001 g/l each) had higher mean visual rating and produced organized structures in 1 month, without callus formation, while explants placed on a MS medium containing 2,4-D + kinetin (0.001 g/l each) produced callus then organized structures. Bulbs developed if these leaf-like structures were placed at 5°C then 24°C. The organized structures could be recultured to produce increased numbers of leaf-like structures. | |
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