ISHS Acta Horticulturae 266: V International Symposium on Flower Buds
POTENTIAL AND LIMITATIONS OF NARCISSUS MICROPROPAGATION: AN EXPERIMENTAL EVALUATION |
| Authors: | W.M. Squires, F.A. Langton |
| Abstract:
An experimental evaluation is reported of 'typical' production figures for the routine micropropagation of a range of narcissus cultivars. The method chosen used 'mini-chips' as the primary explants, and the splitting of bulbing shoots to give continuous further multiplication. Cold-treated bulbils were finally transplanted into the greenhouse. Cultivar differences were shown at all stages of the process. Bulb and explant sizes were implicated as primary determinants of shoot initiation rates, and bulbil weight as an important factor in transplantation success. It was concluded that the average cultivar might be expected to yield c. 1 200 flowering-size bulbs from a single initial bulb in 4–5 years. Limiting factors in the process are discussed. | |
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