ISHS Acta Horticulturae 266: V International Symposium on Flower Buds
HISTOCHEMICAL TEST FOR PRESENCE OR ABSENCE OF CHALCONES IN ANTHERS FROM BULBS OF TULIP CV. APELDOORN PRECOOLED AT 5 °C OR KEPT AT 17 °C |
| Authors: | N. Gorin, R. Sütfeld, J. Tonecki, J.H. Franssen, N. Haanappel |
| Abstract:
Chalcones in anthers from tulip bulbs were detected by suspending 1–3 anthers in several drops of 4–5 M KOH (total volume 0.4 ml). The orange-red pigmentation in the anthers appearing within 10–60 min (positive reaction) denoted the presence of chalcones (2',3,4,4',6'-pentahydroxychalcone, 2',4,4',6'-tetrahydroxychalcone, 2',4,4,6'-tetrahydroxy-3-methoxychalcone). The minimum amount of chalcone detected by the test was 320 nmol, as determined with synthetic 2',4,4',6'-tetrahydroxychalcone. Using this simple histochemical test, we found that chalcones were not present in anthers from bulbs stored at 5 °C for 12 weeks, if storage began before September 22. However, when storage began on October 3rd-5th, chalcones were detected in 4–6 weeks. Moreover, chalcones were always detected in anthers from bulbs cooled at 5 °C for 12 weeks and subsequently kept at 17 °C for one week (transport simulation). Chalcones were also first detected in anthers from bulbs kept at 17 °C (controls) after 2–8 weeks, depending on the date storage began. The earlier storage began, the later was the positive reaction. A negative result could be used to indicate uninterrupted cooling but only for special precooled bulbs cv. Apeldoorn for which cold treatment ended during the first two weeks of December. | |
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