ISHS Acta Horticulturae 265: International Strawberry Symposium
THE USE OF MERISTEM TIP CULTURE TO ELIMINATE VIRUSES FROM STRAWBERRY PLANTS |
| Authors: | V. Kajic, B. Cvjetkovic |
| Abstract:
We tested a technique for virus elimination by meristem tip culture. With this method we have succeeded in producing healthy plants. We took very small pieces (0,2 – 0,3 mm) of apical meristem and we planted them in M.S. nutrient medium (Muraschige and Skoog, 1962) suplemented with organic constituents of glucose and agar (Boxus, 1974). It was a basic medium. On this medium suplemented with 1 mg/1 IBA, O,1 mg/1 6-BAP we succeeded in getting sprouts in 40–50% of cases. The sprouts were aseptically transfered into a basic medium with the adition of IBA 1 mg/1 and 6 - BAP 1 mg/l. After 4–6 weeks of incubation in a climate chamber, the sprouts were multiplied by forming axillary buds so there were 10–15 new sprouts. The sprouts from axillary buds were transfered into basic medium with the addition of 1 mg/1 IBA where they produced roots and leaves. After next 4–6 weeks 95% of thee plants formed roots. Those plants were tested for viruses. It was confirmed that no economicaly major virus diseases were present. | |
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