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ISHS Acta Horticulturae 261: IV International Symposium on Postharvest Physiology of Ornamental Plants

LASER PHOTOACOUSTICS: A NOVEL METHOD FOR ETHYLENE DETERMINATION IN PLANT PHYSIOLOGICAL STUDIES.

Authors:   Ernst J. Woltering, Dane D. Bicanic, F. Harren
Abstract:
A CO2 laser-based photoacoustic method was used to determine the initial changes in ethylene production following emasculation of selected orchid species.

The laser photoacoustic equipment consisted of a line tunable CO2 laser in conjunction with a single pass resonant acoustic cell. The minimum detection limit of the system for ethylene was 0.03 nl/1.

In all tested orchid species an increase in ethylene production was measured within a few hours after emasculation. The time dependent pattern of ethylene production in Cymbidium and Oncidium showed an optimum. In Phalaenopsis and Epidendrum it was characterized by a gradual increase.

The principle of photoacoustics and its application in plant physiological studies is discussed.

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