ISHS Acta Horticulturae 251: III International Symposium on Growth Regulators in Ornamental Horticulture
THE INFLUENCE OF 6-BENZYLADENINE AND THIDIAZURON ON ORGANOGENESIS OF BRASSAIA ACTINOPHYLLA ENDL. CV. AMATE SHOOT EXPLANTS |
| Authors: | T. Badzian, J. Fotyma-Kern, Gary R. Hennen, R. P. Oglesby |
| Abstract:
Shoot explants of Brassaia actinophylla Endl. cv. Amate containing apical meristems were cultured on Murashige & Skoog medium (1962) supplemented with Thidiazuron and 6-benzyladenine at concentrations between 0.5–5.0 mg/l in combination with naphthaleneacetic acid at 0.10 or 0.25 mg/l. At all tested concentrations, thidiazuron exhibited cytokinin activity, strongly inhibited root formation and stimulated shoot bud organization on explant tissue. The lowest thidiazuron concentration tested (0.5 mg/l) was to high for effective shoot multiplication due to induced deformations. 6-benzyladenine was amenable cytokinin to adventitious shoot induction and the optimal concentration in multiplication medium was established as 3.5 mg/l with naphtaleneacetic acid at 0.10 mg/l. Regenerants derived on medium containing optimal 6-benzyladenine dose were easily rooted in vitro and established in soil. | |
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