ISHS Acta Horticulturae 251: III International Symposium on Growth Regulators in Ornamental Horticulture
THE EFFECT OF LIGHT CONDITIONS ON REGENERATION AND LEVEL OF ENDOGENOUS GROWTH REGULATORS IN MUSCARI RACEMOSUM /L./ MILL. BULB-SCALE SECTIONS CULTURED IN VITRO |
| Author: | K. Kromer |
| Abstract:
The effect of light conditions /light and dark/ on regeneration of scale segments of Muscari racemosum has been examined experimentally in vitro. Analysis of the regeneratin effect on MS medium without growth regulators showed an inconsiderable /about 8%/ increase in number of explants which formed the adventitious bulblets in continuous light. Light conditions did not affect the number of adventitious bulblets rising on an explant, but it increased /by about 1.3/ the number of roots. An important difference was noticed in the number of bulblets with emerging leaves, which increased considerably under the influence of light. The activities of endogenous growth regulators were determined by means of bioassays: 1/ at the moment of culture establishment in the tissues of the basal halves of the bulbs, 2/ after four months of culture in tissues of explants growing: a/ in light, b/ in the dark. Determination of growth regulators in investigated basal halves of bulb tissues revealed low activities of auxin- and gibberellin-like substances and increased activity of cytokinin. Light treatment of explants cultured in vitro caused an insinificant increase of auxin activity and slightly decreased the activity of gibberellin. Greater differences were observed in the activities of cytokinins, which was distinctly higher in light-grown explants. | |
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