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ISHS Acta Horticulturae 251: III International Symposium on Growth Regulators in Ornamental Horticulture

TISSUE CULTURE AND MICROPROPAGATION OF LISIANTHUS RUSSELLIANUS HOOK

Authors:   C. Damiano, B. Ruffoni, P. Curir, P. Esposito, F. Massabò
Abstract:
Germination "in vitro" of Lisianthus seeds allows to reduce the time to obtain the plants in condition to be transplanted in soil. Germination percentage was 100% on Brooks and Hough medium (1958) additioned with GA3 and active charcoal.

A protocol of micropropagation is suggested for seedlings or explants taken from adult plants. Multiplication rates ranged from 3.3 to 9.0, the rooting percentage from 84 to 100%.

Furthermore, a method to obtain successfully friable callus, cell culture and regenerations is suggested.

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