ISHS Acta Horticulturae 244: IV International Symposium on Quality of Vegetables
USE OF TISSUE CULTURE IN IMPROVEMENT OF WATERCRESS (RORIPPA NASTURTIUM AQUATICUM L. HAYEK) |
| Authors: | A.C. Gilby, H. Wainwright |
| Abstract:
This paper reports a plant regeneration protocol for watercress using callus derived from somatic tissues. Callus was initiated from explant sources grown in vitro and explants were cultured on MS medium with various levels of auxin and cytokinin for 4 weeks in the dark at 25°C. Auxins tested were NAA and 24D and cytokinins tested were BA and Dropp (thidiazuron). Subsequent regeneration media contained only the cytokinin, and the callus was cultured at 25°C in a 16 hour photoperiod. Dropp was significantly better than BA for shoot regeneration and inclusion of an auxin in the initiation medium was necessary. The nature of the auxin affected both number of shoots and at what stage these were produced. NAA stimulated shoot production from callus on the initiation medium while on 24D shoot production was at its highest frequency at 24–32 weeks. Other influences on shoot regeneration have also been investigated and are discussed. These techniques may also have applications as a source of somaclonal variation or as the basis for an in vitro system for disease resistance selection. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
