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ISHS Acta Horticulturae 236: V International Symposium on Small Fruit Virus Diseases

SOME PROPERTIES OF APPLE MOSAIC VIRUS ISOLATED FROM THIMBLEBERRY IN BRITISH COLUMBIA

Authors:   R. Stace-Smith, J. L. Shier
Abstract:
A group of native thimbleberry (Rubus parviflorus) in British Columbia with virus-like symptoms was found to contain an ilarvirus which was mechanically transmitted to cucumber and Chenopodium quinoa. The virus consists of a single coat protein species of Mr 30 000 and four RNA species of Mr 1.18 x 106, 0.99 x 106, 0.72 x 106 and 0.34 x 106. Virus particles sediment in sucrose density gradients as two major peaks of 82S and 100S and have a buoyant density in cesium chloride of 1.326. Polyclonal and monoclonal antibodies were prepared against the virus and, in serological tests, the thimbleberry virus was identified as a strain of apple mosaic virus.

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