ISHS Acta Horticulturae 236: V International Symposium on Small Fruit Virus Diseases
MULTIPLICATION OF STRAWBERRY CRINKLE VIRUS IN NON-VECTOR INSECTS. |
| Authors: | A.N. Adams, D.J. Barbara |
| Abstract:
Strawberry crinkle virus (SCV), a plant rhabdovirus, acquired by Chaetosiphon fragaefolii from infected seedlings of Fragaria vesca var. semperflorens could be transferred by injection with aphid extracts to the bean aphid Megoura viciae. Three isolates of SCV were injected and all three were detected, by electron microscopy, in extracts of the head and of the body of M. viciae after an incubation period of 8–12 days. The virus was still infective after storage of whole insects at -80°C. SCV was not detected in injected individuals of the leafhopper Euscelidius variegatus, or larvae of the Lepidoptera Mamestra brassicae and Pieris brassicae. Preliminary attempts were made to purify the virus by centifugation of aphid extracts through successive sucrose step gradients but SCV was recovered only after the first centrifugation through 30% sucrose onto a 60% pad. | |
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