ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
ISOLATION OF A VIROID-LIKE RNA ASSOCIATED WITH PEACH LATENT MOSAIC DISEASE. |
| Authors: | R. Flores, G. Llácer |
| Abstract:
A viroid-like RNA was detected in nucleic acid extracts from GF-305 peach seedlings infected with a severe strain of the peach latent mosaic (PLM) agent inducing mosaic symptoms, but not in the extracts from the healthy controls. This viroid-like RNA migrated in denaturing gels between the positions of the circular forms of the 371 base citrus exocortis viroid (CEV) and the 297 base hop stunt viroid (HSV). A second viroid-like RNA, with the same mobility as the previous one, was detected in nucleic acid extracts from symptomless GF-305 peach seedlings infected with a mild strain of the PLM agent. The association of these RNAs with strains of the PLM disease agent strongly suggests a viroid etiology for this malady, as well as for two other ones closely related with it, peach mosaic and peach yellow mosaic. A third viroid-like RNA, migrating in denaturing gels in the same position as the circular form of HSV, was detected in nucleic acid preparations from plants of Jerónimo peach variety of unknown sanitary status in relation to the PLM disease; it remains to be determined if the last viroid-like RNA is associated with a pathological disorder of peach different from the PLM disease. | |
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