ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
COMPARISON OF TWO DIFFERENT PLUM POX VIRUS ISOLATES ON NUCLEIC ACID LEVEL |
| Authors: | E. MAISS, U. TIMPE, W. JELKMANN, R. CASPER |
| Abstract:
Genomic RNA of plum pox virus, severe isolate (PPV-NAT), and purified dsRNA from PPV-AT, mild isolate, were used as templates for the synthesis of complementary DNAs (cDNAs). The generated cDNAs were cloned into various plasmids. Physical maps of the PPV-NAT and PPV-AT genome were constructed by hybridization and restriction endonuclease digestion. The nucleotide sequence of PPV-NAT clones covering about 99% of the whole virus genome has been determined, as well as the nucleotide sequence of the coat protein cistron at the 3'-region of PPV-AT RNA. The respective amino acid sequences of the two coat proteins were compared.
The coat proteins showed 98.4% homology, however PPV-AT revealed 15 additional amino acid residues close to the N-terminal end.
Computer analysis of the coat proteins demonstrated that the additional region of PPV-AT may form a supplementary short Recently the coat protein genes have been prepared for the transformation into plants. This approach may help to establish a cross protection against plum pox virus via the expression of coat protein in transgenic plants. | |
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-helix.
This alteration may possibly be responsible for the ability of aphid transmission, because PPV-AT is aphid transmissible, while PPV-NAT is not.
