ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
DIAGNOSIS OF PLUM POX VIRUS IN INFECTED SYMPTOMLESS TREES OF APRICOT, PEACH AND PRUNUS CERASIFERA SSP. MYROBALANA BY ELISA AND ISEM. |
| Author: | J. Polák |
| Abstract:
The incidence of plum pox virus (PPV) was ascertained by enzyme linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) in apricot, peach, plum, Prunus amygdalo-persica and Prunus cerasifera ssp. myrobalana trees. The Czechoslovak PPV antiserum was used in serological tests. PPV was reliably detected by ELISA in leaves and bark of apricot and peach trees showing virus symptoms on leaves. Virus was detected in bark of shoots, but not in leaves of P. cerasifera ssp. myrobalana with symptoms of mild spotting. PPV was diagnosed by ELISA in apricot, peach, and P. cerasifera ssp. myrobalana, trees, suspected of PPV presence, and in symptomless trees, growing close to diseased ones. PPV was easily detected by ISEM in peach, Prunus amygdalo-persica and plum (cvs Carská, Cacanská lepotica) trees with leaf and fruit symptoms. The virus was also detected in bark of peach GF 305, and of P. cerasifera ssp. myrobalana GF 31 trees without PPV symptoms. | |
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