ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
PROPERTIES OF TWO APPLE CHLOROTIC LEAF SPOT VIRUS ISOLATES |
| Author: | S. Paunovic |
| Abstract:
The differences in biological properties of apple chlorotic leaf spot virus (CLSV) isolates from different sources have been assessed by observing symptom occurrence on a range of indicator plants in the glasshouse and in the field. On the basis of clearly expressed differences, out of 21 isolates tested, obtained from apple, plum, peach and apricot, two were selected for more detailed studies, one (CLSV-LL) from apple, and the other (CLSV-SC) from plum. Studies were conducted to assess whether the differences found in biological properties of selected isolates were related to the differences in their in vitro and serological properties. For that purpose, both isolates were purified using a modification of the Lister and Hadidi (1971) method, and their biophysical properties were determined. Also, the molecular weight of coat protein was assessed by means of SDS-polyacrylamide gel electrophoresis. Rabbit polyclonal antibodies were produced and used subsequently for serological investigations in enzyme-linked immunosorbent assay (ELISA), gel double-diffusion and micro-precipitation tests. The differences between the CLSV-LL isolate from apple and CLSV-SC from plum are underlined and commented on in the paper. | |
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