ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
EPIRUS CHERRY VIRUS, A NEW VIRUS ISOLATED FROM CHERRY IN GREECE |
| Authors: | A. Avgelis, J. Rumbos, B. Marina |
| Abstract:
From a cherry plant showing leaf enations, a virus was isolated and proved to be different from all other known viruses isolated from rasp leaf diseased cherries (Avgelis et al.,1988). The virus, named epirus cherry virus, showed the following properties: Host range: the virus was easily mechanically transmitted to a wide range of plants, among which species belonging to Leguminosae and Solanaceae react very well. For local assay, hosts Chenopodium amaranticolor and Phaseolus vulgaris proved to be the best test plants. No infection was found on Cucurbita pepo and Ocimum basilicum. Aphid transmission: all attempts to transmit the isolated virus by using Myzus persicae failed. Stability in sap: infected plant tissue, infected sap and partially purified virus could be frozen or liophylized without losing infectivity for at least one year. Crude sap of infected Nicotiana benthamiana showed the following properties: T.I.P.: 45°C, D.E.P.: 10-5, L.I.V. 15 days. Purification: Nicotiana benthamiana was the best source for virus purification. The virus was clarified by 25% of chloroform, pelleted by adding 10% PEG (6000 MW) and, upon centrifugation in 10–40% sucrose density gradient, separated into three bands. | |
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