ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
LOCALIZATION OF PRUNUS NECROTIC RINGSPOT VIRUS ANTIGEN IN PEACH TISSUE BY IMMUNOGOLD-SILVER LABELLING |
| Authors: | L. Giunchedi, C. Poggi Pollini |
| Abstract:
Prunus necrotic ringspot virus (PNRV) antigen distribution in sections of systemically infected peaches was examined with light microscopy, using the immunogold-silver labelling technique. Tissue samples were taken from lateral roots, stems and leaf petioles of 6–8 month-old peaches cv. 'Maravilla', propagated in vitro from PNRV-infected peach, and transplanted to pots. Labelling was concentrated in the cortical parenchyma tissue. Occasionally, a few cells in the xylem parenchyma were also specifically labelled. Labelling was never detected in epidermis, in the vascular tissue, in cambium or in pith. Almost all cortical parenchyma cells of the petiole and stem, but only a few root cells showed silver labelling. Within the parenchyma cells the labelling was spread throughout the cytoplasm. On the basis of these observations, it appears that PNRV moves within the plant from cell to cell. | |
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