ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
ELIMINATION OF PVD, NRSV AND CLSV FROM SWEET CHERRIES BY MEAN OF IN VITRO CULTURE |
| Authors: | J.M. Deogratias, F. Dosba, A. Lutz |
| Abstract:
Different methods are proposed in order to obtain virus-free sweet cherries (Prunus avium L.). Three clones infected by NRSV, PDV and CLSV have been cultivated in vitro and used for the elaboration of these techniques. In vitro micropropagation of these clones has been developed to exploit the competition between cellular and virus multiplication. The hormonal composition and content of culture medium seem to play a leading part. Shoot-tip micrografting in vitro and in situ were carried out from this cultivated material and young plants obtained were still virus-free after several months. Thermotherapy and chemotherapy were applied previously to the cultivated material. Axenic plants were obtained by thermotherapy in spite of heavy losses of genetic material. As far as chemotherapy is concerned, different chemical agents were used and the specificity of each virucide was been demonstrated. The therapy was more efficient when the plants were submitted to an intensive vegetative multiplication. The methods to obtain efficient cure of virus infected cherries are discussed. It appears clearly that it depends on the type of genetic material and on the virus(es) to eliminate. | |
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