ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
DETECTION OF THE VIRUS CAUSING GREEN RING MOTTLE DISEASE IN CHERRY BY DIRECT AND INDIRECT ELISA |
| Authors: | K. Zagula Haufler, D.C. Ramsdell |
| Abstract:
Green ring mottle disease which affects Prunus species worldwide is detected by indexing suspect budwood onto the indicator 'Kwanzan' flowering cherry. We recently purified thin, closterovirus-like particles from infected peach and cherry trees. A polyclonal antiserum readily detected green ring mottle virus (GRMV) in infected sweet, sour, duke and oriental flowering cherry budsticks, as well as in atypically symptomatic budsticks, using DAS-ELISA. Absorbance readings for infected samples were nearly 10 times greater than readings for uninfected samples. Indirect protein A ELISA also detected GRMV, although absorbances for infected samples were only about four times greater than absorbances for uninfected samples. No reactions occurred with indirect ELISA when plates were coated directly with test antigens. Green ring mottle can now be detected in two days with ELISA instead of taking several months when indexing is used. | |
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