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ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases

AN ELONGATED VIRUS ASSOCIATED WITH YELLOW MOSAIC OF BABACO

Authors:   B. Marina, P. Giordano
DOI:   10.17660/ActaHortic.1989.235.21
Abstract:
A potexvirus was purified from leaves of babaco plants showing symptoms of yellow mosaic.

Leaves were reduced to powder with liquid nitrogen, mixed with Tris-HCl buffer containing polyclar, bentonite, triton X-100 and mercaptoethanol. In sucrose and cesium sulphate gradients two and one bands were obtained, respectively.

Electron microscope examination of these preparations after negative staining with uranyl acetate, showed elongated particles, the main population of which had length of 575 nm.

All attempts of sap and aphid transmissions to herbaceous hosts and babaco failed.

An antiserum was obtained with the title 1:128.

The virus was decorated by an antiserum to papaya mosaic virus (PMV) of which it could be considered a different strain.

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235_20     235     235_22

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