ISHS Acta Horticulturae 235: XIV International Symposium on Fruit Tree Virus Diseases
REGENERATION OF SHOOTS FROM LEAF DISCS AND STEM MICROCUTTINGS OF FRUIT TREES AS A TOOL FOR TRANSFORMATION |
| Authors: | M. Laimer, A. da Câmara Machado, V. Hanzer, D. Mattanovich, G. Himmler, H.W.D. Katinger |
| Abstract:
Regeneration of plants from single cells and complex explants, either by organogenesis or by somatic embryogenesis, is to be considered a prerequisite for any programme aimed at improving crop quality by transformation via Agrobacterium. Organogenesis was obtained from the commercial cultivars of apple Golden Delicious and Mc Intosh, the local apple varieties Augustkalvill, Ilzer Rosenapfel and Wildtyp, the peach variety Suncrest from a series of stone fruit varieties, Nicotiana clevelandii as a herbaceous host of plum pox virus (PPV) and the pear variety Conference. Expanded leaves and stem microcuttings from sterile shoot cultures grown on modified Murashige and Skoog (MS) media were used. Organogenesis was obtained employing modified MS-media supplemented with benzylaminopurine (BAP) in a concentration range of 4 to 12 mg.l-1 and indolylbutyric acid (IBA) from 1 to 2 mg.l-1, at different ratios ( 1:4, 1:4,5, 1:5, 1:6). While in many conditions only callus appeared on the cut ends of the leaves, in some media globular structures appeared which could be developed into shoots when transferred to other culture conditions. Only very few hormone combinations gave rise to shoots. Close examination of the leaves revealed that most of the shoots appeared on the veins. The histological origin of these shoots is being examined. | |
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