ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
A STRAIN OF CLOVER YELLOW VEIN VIRUS ISOLATED FROM CALANTHE SP. |
| Authors: | N. Inouye, T. Maeda, K. Mitsuhata |
| Abstract:
A potyvirus isolated from Calanthe discolor and C. sieboldi, showing mosaic and/or veinal necrosis on the leaves and flower necrosis, was identified as a strain of clover yellow vein virus (CYVV-C) on the basis of symptoms, host range, properties of particles, serology and immunoelectron microscopy. The virus was transmitted by inoculation with sap to 26 of 52 species from 10 of 14 families. Pisum sativum and Vicia faba developed severe top necrosis and stem necrosis systemically. Twelve cultivars, such as 'Perfected Wales' and 'Fukkoku-Ohza', out of 18 peas tested were susceptible and appeared mosaic and top necrosis, but other 6 cultivars containing 'Wisconsin Perfection' and 'New Season' were not infected. Cucumis sativus developed only local lesions, and Chenopodium quinoa, Nicotiana clevelandii and Gomphrena globos a were infected systemically. It was also transmitted readily by aphid, Myzus persicae, in the non-persitent manner. Sap from Vicia faba was infective after dilution to 10-4 but not 10-5, after 10 min. at 55°C but not 60°C, and after 4 days at 20°C but not 8 days. Filamentous particles, ca. 12 X 750 nm, were found in crude sap. Molecular weight of the coat proteins of CYVV-C and BYMV-O were 34 000 and 34 500 daltons respectively. CYVV-C showed a closer serologically relationshipto to CYVV, appeared to be distantly related to BYMV-O, and differed serologically from BYMV-G. Cytoplasmic cylindrical inclusions with pinwheels and laminated aggregates were observed in the cytoplasms in ultrathin sections of the infected leaf tissues. Additionally small crystalline cytoplasmic inclusions were found in the cytoplasm. In negatively stained extracts, pieces of the cylindrical inclusions and rhombic shaped crystalline inclusions were observed. | |
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