ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
ERADICATION OF ORCHID VIRUSES BY CHEMOTHERAPY FROM IN-VITRO CULTURES OF CYMBIDIUM |
| Authors: | J. ALBOUY, C. FLOUZAT, C. KUSIAK, M. TRONCHET |
| Abstract:
In Orchid cultures two viral diseases caused by Odontoglossum Ring Spot (ORSV) and Cymbidium Mosaic (CyMV) have been reported to limit the production. Obtention of virus free orchids by, meristem tip culture has proved to be often inadequate. Different chemicals such as Virazole have been reported as means of freeing plant culture in vitro from viruses. In order to increase the efficiency of eradication of ORSV and CyMV chemotherapy based on the incorporation of Virazole in the solid medium culture has been investigated on Cymbidium protocorms cultured in vitro Factors such as growth regulators and active charcoal are of critical importance. Best results are obtained in shortening the time of transplantation (18 days) allowing a good excision of Cymbidium new protocorms. After 5 subcultures of 18 days in presence of 25 ppm of Virazole we obtained 95 % virus free plantlets. | |
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