ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
PRODUCTION OF PLANTS FREE OF VIRUS AND PREVENTION OF REINFECTION |
| Author: | R.K. Horst |
| Abstract:
The production of ornamental plants tested to be free of specific viruses has been utilized commercially since the early 1950's. The methodologies for detection of virus and virus-like pathogens has dramatically changed in recent years. Some of the changes include the following new procedures 1) enzyme linked immunosorbent assay, 2) polyacrylamide gel electrophoresis, 3) cDNA probes, 4) various methods associated with electron microscopy, 5) inclusion bodies, 6) epifluorescent microscopy, and 7) improved uses of bioassays. All these procedures have advantages and disadvantages which are outlined and discussed. Moreover, the methodologies to free plants of virus and viruslike pathogens have dramatically changed. Improved methods for tissue culture provide new lines of approach for obtaining plants free of specific viruses and for maintaining nuclear plant material. To acquire adequate production plant numbers for sales and to maintain production plants free from virus and viruslike pathogens require special handling and sanitation procedures. These production methods are discussed relative to the various commercial ornamental crops for which virus tested programs are available. | |
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