ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
QUANTITATIVE USE OF THE ELECTRON MICROSCOPE DECORATION TECHNIQUE FOR PLANT VIRUS DIAGNOSTICS |
| Author: | R.G. Milne |
| Abstract:
The decoration technique, as has been known for ten years now, can be used to titrate antisera and so arrive at quantitative estimates of degrees of serological relationship between virus isolates. However, decoration is most often employed only in qualitative tests yielding less precise information. The virtues and simplicity of quantitative decoration are therefore illustrated. The method is rapid (results from start to finish within 60 min), economical of antigens and antibodies, and resistant to the generation of false results. Prior elaboration of reagents is not necessary. If combined with immunosorbent electron microscopy (ISEM), quantitative determinations can be made on very small amounts of crude extract containing low concentrations of virus. Thus, if an electron microscope is available, the method is well adapted to research applications and to routine testing on a limited scale. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
