ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
CYTOPATHIC STRUCTURES ASSOCIATED WITH ROBINIA MOSAIC VIRUS, A STRAIN OF PEANUT STUNT VIRUS |
| Authors: | Z. Štefanac, M. Wrischer, N. Bezic |
| Abstract:
Leaf cells infected with robinia mosaic virus from particular areas with well-formed symptoms have been investigated by light and electron microscopy for intracellular changes. Light microscopy of living tissue of Chenopodium quinoa and Nicotiana megalosiphon showed large crystalline inclusions in the form of different polyhedral bodies in some epidermal and mesophyll cells. These inclusions resembled the large virus crystals with hollow centres often found within the central vacuole in cucumber mosaic virus (CMV) infections. The form of the polyhedral crystals and their frequency were influenced by external conditions and not by the host plant as previously recorded. The sequence of setting of the virus in formation of polyhedral crystals is discussed. In thin sections of infected C. quinoa accumulations of small crystals built of doughnut-like virus particles were seen in the vacuole. Small vesicles associated with the tonoplast, appearing to be a general characteristic of cucumoviruses, were often observed. Some other cytopathic structures typical of CMV were also found. Darkly stained rough endoplasmic reticulum suggested specific activity in infected cells. | |
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