ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
THE DETECTION OF DASHEEN MOSAIC VIRUS USING THE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) |
| Authors: | B.C. Rodoni, J.R. Moran |
| Abstract:
An antiserum to a Victorian isolate of dasheen mosaic virus (DMV) was produced and used to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of DMV. Unreliable results were obtained using standard ELISA buffers in both healthy and DMV infected sap of Dieffenbachia picta and Philodendron selloum. High absorbance readings occurred for sap of both healthy and infected D. picta, whereas uniform low readings occurred for sap of healthy and infected P. selloum. In subsequent experiments reliable results were obtained for both species following the addition of the sugar mannose and polyvinylpyrrolidone (PVP) to a tris buffer of high ionic strength containing Na2 EDTA. A reliable ELISA was established for DMV using both the alkaline phosphatase and urease enzyme systems. The results showed that the optimum extraction buffer was species specific. This work highlights the potential difficulties of developing ELISA systems for individual host/virus combinations. | |
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