ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
A POTYVIRUS INFECTING RANUNCULUS ASIATICUS |
| Authors: | M.S. Elliott, M. Gallegati, F.W. Zettler, N.-J. Ko |
| Abstract:
A virus (RV), possibly "ranunculus mottle" (Laird and Dickson, 1965), was detected in Ranunculus asiaticus plants grown from tuberous roots in southern California. RV was transmitted manually to R. asiaticus seedlings and to larkspur (Consolida ambigua), but not to Brassica rapa, Cucurbita pepo, Nicotiana benthamiana, Zinnia elegans or to 20 other species in 9 families. RV was transmitted by Myzus persicae aphids allowed 20–60 sec acquisition probes. Cytoplasmic inclusions were seen in tissues stained in calcomine orange/Luxol brilliant green and examined by light microscopy. Cylindrical ("pinwheel") inclusions were also seen in extracts negatively stained in 2% uranyl acetate and in ultrathin sections of samples prepared for electron microscopy. Of 193 negatively stained virus particles measured, 78 had a modal length of 707 nm and 19 a modal length of 1471 nm (ranges of 651–769 and 1358–1556 nm). In unilateral sodium dodecyl sulfate immunodiffusion tests, homologous precipitin lines of watermelon mosaic virus 2 spurred over those of RV. RV did not react against antisera to turnip mosaic, bean yellow mosaic, zucchini yellow mosaic or to 8 other potyviruses. | |
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