ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
NATURAL INFECTION OF TULIPS WITH TURNIP MOSAIC VIRUS AND ANOTHER POTYVIRUS ISOLATE DISTINCT FROM TULIP BREAKING VIRUS IN THE U.S.A. |
| Authors: | J. Hammond, Gary A. Chastagner |
| Abstract:
A potyvirus ('Wa tulip virus', WaTV) isolated from tulip infected several herbaceous species including Nicotiana benthamiana, and was serologically related to bean yellow mosaic virus (BYMV), as well as to tulip breaking virus (TBV), tulip chlorotic blotch virus (TCBV) and turnip mosaic virus (TuMV). During testing of many tulip cultivars for TBV using an indirect antigen-coated form of ELISA with a panel of monoclonal antibodies (MAbs) and polyclonal TBV, TCBV and WaTV antisera, several plants showed a spectrum of reactivity with the MAbs and polyclonal sera that differed from typical TBV infections. Additional potyvirus isolates infecting N. benthamiana were obtained by sap inoculation from some of the plants with atypical MAb and polyclonal sera reactions. These isolates were identified as TuMV on the basis of host range and serology. Further comparison was made of WaTV and one tulip isolate of TuMV (TuMV-L2) with BYMV and TuMV (ATCC PV134) by nucleic acid hybridization and by ELISA with both MAbs and polyclonal antisera. These tests showed that WaTV is related to both TuMV and BYMV, but more closely to BYMV, and that TuMV-L2 was distinguishable from TuMV PV134 by nucleic acid hybridization. The other isolates from tulip were also identified as TuMV by nucleic acid hybridization and double antibody sandwich ELISA. TCBV was found to be so closely related to TuMV that it may be regarded as an isolate of TuMV. | |
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