ISHS Acta Horticulturae 234: VII International Symposium on Virus Diseases of Ornamental Plants
OCCURENCE AND IDENTIFICATION OF CARNATION NECROTIC FLECK VIRUS IN CZECHOSLOVAKIA |
| Authors: | V. Mokrá, J. Mertelík, B. Götzová |
| Abstract:
A new disease of carnations /Dianthus caryophyllus/ was observed in several establishment especially on imported cultivars belongin to the new type of hybrids with higher degree of tolerance to Fusarium wilt. The symptoms were yellow or purple partially necrotic streaks or flecks in older leaves which dried later. It was proved by ELISA tests that all these plants were infected by carnation mottle virus, some of them also by carnation etched ring virus. In leave samples of carnation plants with necrotic symptoms checked by electron microscopy also flexuous filamentous particles varying in lenght from 1 100–1 600 nm were commonly detected, but mostly in low concentration. The presence of particles resembling virions of Closterovirus group was confirmed in carnation plants of 27 cultivars. The particles were never found in symtomless plants. For serological identification carnation necrotic fleck virus antiserum prepared by I.N.R.A. in Antibes and supplied by Sanofi, France was used in ELISA tests and in immunoelectron microscopy /IEM/. With some varieties we got positive reactions in ELISA tests for carnation necrotic fleck virus /CNFV/. We proved that CNFV was unregulary distributed in the plants. In IEM the trapping of virions was very effective. When using decoration some particles were fully decorated, some partially decorated and some undecorated. Further experiments to clarify this phenomenon are in progress. | |
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