ISHS Acta Horticulturae 23: I International Symposium on Flowerbulbs
EFFECTS OF TEMPERATURE, LIGHT, SUGARS AND GIBBERELLIN A3 ON THE GROWTH IN VITRO OF TERMINAL BUDS OF TULIP |
| Author: | J. van Bragt |
| Abstract:
The growth of terminal buds of tulips (holding a complete flower) isolated from bulbs of the cv. 'Apeldoorn' was investigated. The bulbs were pre-treated by dry-storage at 5°C for 0, 6, or 12 weeks. The buds were grown on a basic medium containing sugars and gibberellin A3 (GA3). The growth of isolated buds was promoted by increasing the duration of the 5°C pre-treatment and retarded by cultivation at low temperature, and the presence in the medium of fructose, glucose or sucrose. Darkness gave a slight retardation of growth. Growth was promoted by GA3. In most cases growth promotion was accompanied by early flower blasting. Flowering was obtained with explants from bulbs treated at 5°C for 6 weeks and cultivated in light, first at 9°C and then at 18°C, on a medium containing sucrose and GA3. | |
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