ISHS Acta Horticulturae 23: I International Symposium on Flowerbulbs
STUDIES ON NUCLEAR PROTEINS OF TULIP: NUCLEAR ISOLATION AND PROTEIN FRACTIONATION |
| Author: | W.F. Sheridan |
| Abstract:
Tulip leaves of cv. 'Golden Age' were disrupted by blending in dry ice. Nuclei were isolated after dehydration in cold acetone by centrifugation in carbon tetrachloride-cyclohexane mixtures of varying densities. The purest nuclear fraction was obtained at a density between 1. 450 and 1. 470 at 0°C. DNA assays indicate about a five fold purification of nuclei Soluble proteins, histones, chromosome residual proteins, and nuclear residual proteins were prepared and analyzed for protein and DNA content. | |
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