ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
LIGHT QUALITY AND THE CONTROL OF SHOOT LENGTH IN WOODY ORNAMENTAL PLANTS GROWN IN VITRO |
| Authors: | Colin R. Norton, T. Herrington, M. E. Norton |
| Abstract:
Shoot tip explants of Potentilla, Rhododendron 'Lee's Dark Purple', Spiraea and Vitis vinifera were cultured in vitro on Linsmaier and Skoog medium with 0.5 mg l--1 benzyladenine. The pH was adjusted to 5.6 except for Rhododendro for which the pH was 4.8. Cultures were incubated in modified growth chambers using light pipe and metal halide or high pressure sodium illumination. The control used fluorescent tubes. Shoot length was greater in Potentilla and Spiraea when metal halide or high pressure sodium light sources were used; however, in Rhododendron and Vitis, a When explants were illuminated with red light, then longer shoots were produced than under white light. Length of shoots under blue light was less than for either red of white light. If cytokinin levels were not limiting then both blue and white light gave shoots of similar length. | |
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