ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
THE ROLE OF SEASON IN DETERMINING EXPLANT SUITABILITY FOR IN VITRO CULTURE OF PRUNUS AND SPIRAEA |
| Authors: | M. E. Norton, Colin R. Norton |
| Abstract:
Shoot tip explants of Prunus and Spiraea were cultured in vitro at monthly intervals during spring, summer and autumn on Linsmaier and Skoog medium containing benzyladenine for shoot induction or indolebutyric acid for root formation. Cool white fluorescent lighting was given for 16 hours per day for shoot formation. For root formation, a one-week dark period followed by 3 weeks in light was given. Mean shoot number varied with the month of initial introduction to culture in both species and at all BA concentrations. A significant difference in shoot number between seasons was demonstrated (p<0.001). Shoot number peaked in May and June and again to a lesser extent in September. In Spiraea, a BA concentration of 1.0 mg l-1 gave most shoots in March, April, July, August and October. In May, June and September, 0.5 mg 1-1 was the optimal BA concentration for shoot formation. In Prunus, 0.5 mg l-1 gave most shoots in all months except March and October when 1.0 mg l-1 was optimal. Modal shoot length did not change between May and September but was reduced in March, April and October. A considerable increase in shoot number occurred when August-cultured explants were subcultured; shoot number increased to a lesser extent when May-cultured explants were subcultured. Root number also varied with month of culture and was greatest in May and June for both species. | |
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